@article{Dinçer_2023, title={Determination of optimal plant growth regulators for breaking seed dormancy and micropropagation of Sorbus aucuparia L.}, volume={29}, url={https://balticforestryojs.lammc.lt/ojs/index.php/BF/article/view/679}, DOI={10.46490/BF679}, abstractNote={<p><em>Sorbus aucuparia</em> L. is an important forest tree used in the reforestation of high-altitude mountainous areas, which carries significant pharmaceutical, industrial, ornamental, food, and ecological properties. However, the seed propagation and micropropagation of mature trees of <em>S. aucuparia</em> L., presents various difficulties which are integral aspects for the re-propagation and breeding of novel cultivars. In this study, we isolated the mature embryo-containing seeds of selected varieties of <em>S. aucuparia</em> L. from their seed coat and used them directly as explants <em>in vitro</em> to investigate and determine the optimal dose of cytokinin in breaking seed dormancy and micropropagation. 3 mg/L of benzyl adenine (BA) in addition to different concentrations and combinations of kinetin (0.5, 1, and 2 mg/L), indole-3-butyric acid (IBA) (0, 1, 0.5, and 1 mg/L), and 1-naphthalene acetic acid (NAA) (0, 1, 0.5, and 1 mg/L) were applied within a Murashige and Skoog (1962) (MS) medium at germination inhibition, shoot elongation, and shoot proliferation. In the MS mediums containing BA and kinetin, germination was achieved at the end of 1 week, and shoot proliferation was achieved at the end of 3 weeks. The most successful germination (96%), tallest shoot length (mean 5.1 cm), most shoot proliferation (mean 7.2 pieces), and number of nodes (mean 9.7 pieces) were identified in the MS containing 3 mg/L BA and 1 mg/L kinetin. Direct root formation with shoot elongation occurred in 25% of explants which germinated in the MS medium. For shoots propagated without roots in this medium, 62% of these achieved rooting at the highest dose of 1 mg/L indole-3-acetic acid (IAA) using a two-stage rooting method. Rooted shoots were successfully transferred to an <em>ex vitro </em>medium. These results provide a basis for breaking seed dormancy of selected <em>Sorbus</em> L. genotypes quicker, leading to more effective clonal production.</p> <p><strong>Keywords:</strong> <em>Sorbus aucuparia</em> L., rowanberry, tissue culture, seed dormancy, micropropagation, <em>in vitro </em></p>}, number={1}, journal={Baltic Forestry}, author={Dinçer, Deryanur}, year={2023}, month={Apr.}, pages={id679} }